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Then, the cells were harvested and lysed with 0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5 to afford a protein free lysate
The stability of BPC-157 under harsh conditions is a key enabler of its broad "pleiotropic" effects across multiple organ systems, as it can reach systemic circulation or act locally within the gastrointestinal tract without significant loss of integrity
GHK-Cu is a naturally occurring copper peptide that stimulates collagen production, supports wound healing, and protects against oxidative damage
It enhances the expression of genes coding for Atg proteins (Reference Koji, Spremo and orievski42)
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