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Cell cultures were cooled to 18C and expression of the GS-like proteins of interest induced with addition of IPTG to a final concentration of 1 mM

Vitrified specimens and data collection Holey TEM grids (Ultrafoil R1.2/1.3 Au 300 gold foil grids, Quantifoil GmbH) were pre-washed with chloroform prior to glow discharged

Further analyses revealed that changes in blood GSH were highly correlated with those in erythrocytes, and changes in lymphocyte GSH were significantly correlated with those in both blood and erythrocytes

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Dirven HAAM, van Ommen B, van Bladeren PJ: Involvement of human glutathione S-transferase isoenzymes in the conjugation of cyclophosphamide metabolites with glutathione