Briefly, bEnd.3 cells were preseeded in 96-well plates (Cell Carrier Ultra, PerkinElmer, 1 10 4 per well) and cultured at 37 C for 24 h
Meanwhile, in RAAS, ACE's conversion of angiotensin I to angiotensin II, which has a potent vasoconstrictor impact, may cause the adrenal cortex to generate more aldosterone, both of which raise blood pressure
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doi: 10.1172/JCI110908
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