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Of this latter category, fusion proteins have emerged as a prominent method for purification

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We use single-use vials and needles and store everything to the manufacturers specification

In vitro experiments To plate cells for infection, aliquots of BMDMs were thawed on ice, diluted into 9 ml of DMEM, centrifuged in an Eppendorf 5810 R at 1,200 RPM (290 x G) for 5 min, and the pellet was resuspended in 10 ml BMDM media without antibiotics