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Role of TNF-alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis
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For the measurement of total chlorophyll of leaves, a 0.5 g leaf sample was homogenized in 5 mL acetone (80% v/v), followed by centrifugation at 10,000 g for 8 min, after which the optical density was measured at 663 and 653 nm for Chl a and Chl b , respectively, using a spectrophotometer (Beckman 640 D, USA) by following the method of Arnon 34
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