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To determine the likelihood of SyeA orthologues from diverse Buchnera being substrates for a T3SS, a total of 103 SyeA orthologues was run in BastionX, with the common use parameter for v.2.0 selected (see Supplementary Dataset 2 at Figshare 17 )

The trial design included a core study phase consisting of a four-week screening period, followed by a four-week baseline phase where participants received only a placebo in a single-blind manner, and then a 24-week randomized treatment phase, where participants received either bremelanotide or placebo [1]