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Saline has no preservative of any kind

Comparing the efficacy and tolerability of a new daily oral vitamin B12 formulation and intermittent intramuscular vitamin B12 in normalizing low cobalamin levels: a randomized, open-label, parallel-group study

Lift it away from the underlying muscle and bone

Allow the vial and the bacteriostatic water to reach room temperature, swab both rubber stoppers with alcohol, then inject the diluent slowly down the inner glass wall rather than onto the lyophilised cake, and swirl gently until fully dissolved

Bacteriostatic means that it prevents (stops) the growth and reproduction of bacteria without necessarily killing them outright