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16 Additional genetic alterations, including frameshift mutations in ATG2B, ATG5, ATG9B , and ATG12 17 as well as cancer-specific splicing variants of ATG5 , further impair autophagy and contribute to tumor initiation

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Mutations in the gpt gene were quantified in both wild type and OGG1+ cells by sequencing after 13-15 days following 400 kJ/m 2 UVA irradiation G:C-A:T mutations in UVA-irradiated OGG1+ cells were completely eliminated G:C-A:T mutation frequency in wild type cells increased from 1.8 mutants/million cells to 3.8 mutants/million cells following irradiation indicating incorrect repair or lack of repair of accumulated 8-oxo-dG Elevated levels of OGG1 was able to prevent G:C-A:T mutations, while the OGG1 levels in wild type cells was insufficient, leading to an increase in mutants (demonstrates inadequate repair leading to mutations) Xeroderma pigmentosum complementation group A (XPA) knockout (KO) and wild type TSCER122 human lymphoblastoid cells were transfected with TK gene-containing vectors with no adduct, a single 8-oxo-dG, or two 8-oxo-dG adducts in tandem (Sassa et al., 2015)

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