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IGF pathways are involved in both anabolic signaling and tumorigenesis, highlighting the importance of dose control and periodic cycling. Pollak, Nature Reviews Cancer Desensitization Overuse can lead to IGF1 receptor downregulation, blunting the bodys natural anabolic response over time

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JCRB1819) cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 7.5% (v/v) FBS and kanamycin (50 g/mL) in 5% CO2 and 95% air at 37 C

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