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This depends on your dose and is something you'll discuss with your clinician and clinical team in training

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For a 70 mg glow blend vial, adding 3 mL of bacteriostatic water is standard

The role of isovalerate in psychiatric disorders is however uncertain, and there are some studies evidencing that an increase of this component is directly correlated with depression and cortisol levels, being able to interfere with synaptic neurotransmitter release after crossing the BBB [259]