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These tests include SDS-PAGE and HPLC

J Exp Med 2015

Key Benefits: Why Glutathione

So people at greatest risk for deficiency include those with low stomach acid (e.g., older adults and people on proton pump inhibitors or antacids) or low IF production (known as pernicious anemia), people on medications that interfere with B12 absorption (such as metformin), people who have had gastric surgery, those with GI conditions that impair nutrient absorption (like celiac or Crohn's disease), and people on vegan and vegetarian diets

Furthermore, GST fusion proteins are susceptible to TG2-mediated aggregation, independent of the specific fused protein that is used