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As the first triple-receptor agonist targeting GLP-1, GIP, and glucagon receptors simultaneously , it represents a major evolution beyond single- and dual-agonist incretin therapies

Quantitative RT-PCR RNA was extracted from cell lysates using RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA) and was reverse-transcribed using QuantiTect Reverse Transcription Kit (Qiagen, Chatsworth, CA, USA)

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Regulatory note: Injectable/IV NAD+ is not FDA-approved (or approved by any major regulator) for energy, metabolic or longevity uses