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The next day, acetone was removed post-centrifugation at 17,000 g at 4 C, washed twice with 80% ice-cold acetone, and resuspended in digestion buffer consisting of 3 M urea and 100 mM HEPES at pH 8.0 such that the final protein concentration was 1 g/L

Acneiform eruptions caused by vitamin B12: a report of five cases and review of the literature

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In the United States, by contrast, the Dietary Supplement Health and Education Act of 1994 classifies herbal products as dietary supplements, a product category that does not require pre-approval

The outcome of the study suggested that the lethal dose of the aqueous extract was higher than 2000 mg/kg in female rats [53]