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Briefly, proteins were subjected to sodium dodecyl sulfate-10% polyacrylamide gel electrophoresis (SDS-10% PAGE) under reducing conditions in Laemmlis buffer system followed by transfer to nitrocellulose membranes 19

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Its maximal activity is about five times the rate of glucose consumption by the RBC, but it is subject to feedback (allosteric) inhibition by its product Glc-6-P