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The classic: The serum vitamin B12 blood test If you suspect you have a vitamin B12 deficiency , the first and most common step is a serum vitamin B12 blood test

A subcutaneous injection, which goes into the fatty tissue just beneath the skin, provides a slower, more gradual release

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As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only