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The digest was filtered through a 70-m mesh and centrifuged using a 28.7% Nycodenz gradient (Nycomed PharmaAS, Oslo, Norway)
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Thus, the greater understanding of the molecular regulatory network involved in the regulation of Zn deficiency response and Zn homeostasis would contribute to Zn phyto-availability by developing crops with an increased content of bioavailable Zn in plants, which has been considered as most cost-effective methods for combating Zn deficiency in plants and malnutrition problem in humans
Fatemi SH, Aldinger KA, Ashwood P, Bauman ML, Blaha CD, Blatt GJ, et al