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For rat 3-NP study, one set of serial sections (210 m apart) immunostained using a mouse antibody against the neuronal marker NeuN were measured for areas of 3-NP-induced NeuN loss to determine the lesion volume using the cavalieri principle as estimator in the same stereology software program

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Experimental and therapeutic medicine

2 History of probiotics The functional concept of probiotics originates from ancient fermentation practices that predated the scientific discovery of microbes ( The modern scientific framework emerged in the early 20th century with Metchnikoff, who proposed that yogurt-derived bacteria could beneficially modulate the gut microbiota and improve health, opening the way for systematic probiotic research and strain isolation from diverse sources, including non-traditional niches such as fish gallbladders ( Lactobacillus , Bifidobacterium , and several other genera

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