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Interestingly, a recent screening of more than 20,000 oocytes revealed that most of trisomy 21 cases are due to errors occurred at maternal MI or to sequential MI and MII errors, and should be therefore linked to recombination errors that occurred or initiated during the fetal development of the mother in the maternal grandmother's body (Kuliev et al., 2011)

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The stimulated cells were fixed with 4% PFA in PBS for 30 min and stained with 0.2% crystal violet in 20% methanol

A: Benzyl alcohol is a bacteriostatic agent, meaning it slows or prevents bacterial growth without destroying microorganisms