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LC-MS was a common method to detect TMAO which was easy to operate in clinical application

The MICs were determined as the lowest concentrations of GSSG or antibiotics with no visible bacterial growth after 1624 h of co-incubation at 37 C

We found no differences between the preparation conditions tested nor an effect of time to deproteinization on stability of total GSH, suggesting that use of either heparin or EDTA during sample collection can prevent enzymatic degradation of GSH when samples are stored at 80 o C

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