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The final step is deprotonation (Step 6, arrows K and L) to give the neutral primary amide

To sum things up simply, Carnitine plays an essential role in energy production

In vivo, zinc deficiency does not independently induce ER stress, yet under pharmacologically induced ER stress conditions, zinc-deficient mice exhibit heightened activation of the pro-apoptotic p-eIF2ATF4CHOP axis, accompanied by apoptosis, steatosis and liver injury

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Afterwards, 150 L DMSO was added to each well to dissolve the crystals constituted by the living cells and the absorbance at 490 nm was tested to get the relative cell viability