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The results of this study clearly show that childhood asthma is associated with significant decreases in various components of both enzymatic and nonenzymatic antioxidant defenses [45]

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These findings reinforce the need for integrated monitoring strategies employing bioindicators, stricter regulatory enforcement, and targeted risk management efforts to address the impacts of heavy metal pollution

2.3 Determination of biochemical indicators 2.3.1 Determination of glutathione levels in heart tissue The measurements of oxidized (GSSG) and reduced glutathione (GSH) were performed in the heart homogenates with Ellmans reagent ( 2.3.2 Measurement of the H 2 S content The content of H 2 S was determined in the suspension of heart mitochondria as described previously with modifications ( 2 S will be used to represent all sulfides