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Samples were incubated at room temperature for 1 h, vortexing intermittently, and then quenched by the addition of hydroxylamine (5 L of 5% w/v in HPLC water per sample) and incubated for 15 min at room temperature

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Consistent with the MDA results, we also found a concomitant qualitative increase in 4-HNE protein adducts with age that was suppressed by both Lip-1 and SIH treatments (Figure 2F)

As a result, even the most determined individuals can find themselves battling against their bodies