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Park KK, Liu K, Hu Y, Smith PD, Wang C, Cai B, et al

RhoGTPases at the synapse: an embarrassment of choice

376,377 Obesity is a metabolic disorder caused by genetic, environmental, and other factors, which is related to a variety of metabolic diseases, such as diabetes, hypertension, cardiovascular, and cerebrovascular diseases

An equal volume of 2 urea sample buffer (150 mM Tris pH 6.8, 6 M Urea, 6% SDS, 40% glycerol, 100 mM DTT, 0.1% bromophenol blue) was added to the lysates and heated at 65C for 5 minutes