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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration Following these practices helps support consistent and reproducible laboratory results

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The redox potentials of T[SH] 2 (2,242 mV) and GSH (2,230 mV) are very similar, though T[SH] 2 (pKa 7.4) and GSH (pKa 8.7) exhibit dramatic differences in their pKa values (Moutiez et al., 1994)

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