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Glutathione can be broken down in the bodys digestive system

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Cells were then fixed with 4% PFA for 10 min at RT, permeabilized with 0.3% TritonX-100 in PBS for 5 min at RT and incubated with 10% horse serum in PBS/1% BSA for 1 h

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Primary antibodies included anti--Catenin, anti-GSK3, anti-phospho-GSK3, anti-GAPDH, and anti--tubulin (Boster Biological Technology, Pleasanton, CA, United States), anti-Bcl2, anti-Bax, and anti-Cyclin D1 (Abcam plc, Cambridge, United Kingdom), anti-Akt and anti-phospho-Akt (Cell Signaling Technology, Danvers, MA, United Ststes), anti-Wnt10b, anti-CDK2, anti-CDK4, and anti-P21 (ABclonal, Wuhan, China), and anti-ki67 (Proteintech, Wuhan, China)