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Assays were performed in triplicate for each sample on a Roche Lightcycler 480 system (Roche, Basel, Switzerland) using Realtime Ready DNA probes Mastermix supplemented with ResoLight Dye (Roche) according to the manufacturers protocol

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[286] refined this approach for NAFLD therapy by incorporating charge-reversal mechanisms to concurrently modulate macrophage-mediated inflammation and hepatocyte lipid balance, thus addressing the constraints of single-target interventions

Those antibodies could make future doses less effective, increase the risk of future reactions, or, in some therapeutic proteins, interfere with related natural proteins in the body

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