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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

Longevity:Product Expiry Date: March 2026

doi: 10.1128/iai.00069-18 33 DingQ.GaskaJ

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Given the close correlation between global population growth, increasing aging, and tooth loss rates, periodontitis presents a significant public health challenge (Kassebaum et al., 2014)