Howard, M
Dark circle improvement effect (4.05%)
Each variable was scored for each sample slide, and we used a common scoring system where [0] = no alteration, [1] = mild changes, [2] = moderate changes, and [3] = severe changes ( 2.8 Immunohistochemical analysis Paraffin sections from different groups of liver tissues from rats were stained by immunohistochemistry (IHC) according to the manufacturing protocol using Anti-caspase 3 antibody (ab4051), at dilution 1:1,000, Anti-Bax antibody (ab32503), at dilution 1:250 and Anti-Bcl-2 antibody (ab182858), at dilution 1:500, (abcam, Cambridge, United Kingdom)
Oxidative Stress Theory According to the oxidative stress theory, intra-epidermal buildup of reactive oxygen species the most well-known of which is hydrogen peroxide (H2O2), whose concentration can reach up to one millimole is the primary cause of vitiligo
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