Afterwards, cells were reacted overnight at 4 C with the appropriate primary antibody diluted in the IF antibody dilution buffer (1X PBS, 1%, BSA and 0.3% Triton X-100)
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The compounds were injected during the assay, and the measurement periods for examining the OCR and ECAR were 2 h
TMAO has been shown to affect macrophage scavenger receptor expression, promoting foam cell formationa hallmark of atherosclerosis [1][5]