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Total RNA was extracted from frozen tissue samples using TRIzol reagent, followed by conversion to complementary DNA (cDNA) using SMART Scribe Reverse Transcriptase (Clontech Laboratories, Inc., a Takara Bio Company)

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Research Applications and Mechanistic Considerations Cagrilintide's selective amylin receptor agonism makes it valuable for studies isolating gastric motility effects from incretin-mediated insulin secretion