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For immunofluorescence, the primary antibodies used were monoclonal [8G9] to GLP-1, monoclonal SST antibody (#MAB2358), and monoclonal Human/Mouse Ghrelin Antibody (R&D systems #MAB8200), and the secondary antibodies were Alexa Fluor 488 AffiniPure Donkey Anti-Rat IgG and Cyanine Cy3 AffiniPure Donkey Anti-Mouse IgG from Jackson ImmunoResearch (Europe Ltd.) and were used at a final dilution of 1/300

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If used with medicines that also lower sugar, the risk of hypoglycemia increases, especially with: Insulin Sulfonylureas (e.g., glipizide, gliclazide, glimepiride) If you use any of these, your clinician may want closer monitoring

Neurology 78 , 13231329 (2012)