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Consistently, the protein-lipid overlay assay showed that SNX2a, like SNX1, binds to PI(3)P, which is essential for its membrane-binding ability (Fig

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The membrane was blocked for 2 h with 5% nonfat dry milk in TBS-Tween (TBS-T: 50 mM Tris-HCl, 150 mm NaCl, and 0.1% Tween 20 (pH 7.4)), and then incubated overnight at 4 C with 1.78 g/ml GLP1R-specific antibody (MAb 3F52) 24 in TBS-T

Lee, M.J