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Liraglutide was found to activate osteoblast proliferation by promoting the Wnt signaling pathway and p-AMPK/PGC1 signaling pathway, and inhibit osteoclast activation by suppressing the OPG/RANKL/RANK signaling pathway through anti-inflammatory, antioxidant, and anti autophagic pathways, thereby reversing bone loss ( There are certain limitations of this study that should be noted

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After incubation, the suspension was centrifuged at 2000 rpm for 5 min, and the supernatant was measured at 540 nm using a microplate reader