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CXCL13 and S1P) were unchanged in Gclc -deficient mice when compared to control littermates (Supplementary Fig

Following H 2 O 2 treatment, cells were collected by centrifugation, resuspended in serum-free medium containing 10 M DCFH-DA, incubated for 30 min at 37C, washed with PBS to remove unreacted dye, resuspended in PBS and was detected at 485 nm excitation and at 535 nm emission using flow cytometry

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