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If a vial has been opened or the seal is compromised, the powder may absorb ambient moisture, effectively changing the amount of "dry" peptide before you even add water

Sekhar RV, Patel SG, et al

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After first centrifugation, plasma and buffy-coat layer were removed, and RBCs were washed twice by centrifugation at 400 g for 3 min (Centrifuge ELMI-50CM, Riga, Latvia) and resuspended in HEPES-buffer

In contrast, the no loading dose regimen resulted in a gradual increase, reaching a median vitamin B12 concentration of 717 pmol/L after 90 days