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After washing three times with PBS, cells were fixed with 1 mL 4% paraformaldehyde for 15 min, permeabilized with 1 mL 0.1% Triton X-100 for 10 min at room temperature, and blocked with 1 mL 5% BSA for 30 min

5-GATCGGATCCGCAGGATCAGCCATGGTAGA-3 ORF encoding human GSTP1 was synthesized by RT-PCR from HaCaT cells RNA using First strand cDNA synthesis kit (Amersham Biosciences, Piscataway, NJ) with the following specific primers: GSTP1 FW

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