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In order to gain insights into biological pathways associated with increasing emphysema severity within a patient and explore computational strategies for identifying COPD therapeutics, we obtained paired samples from eight regions at regular intervals between the apex and base of each explanted lung from six patients with severe COPD (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage IV) and two donor lungs
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Acknowledgements The authors sincerely thank all participants and their families/caregivers for their valuable contribution to this research
The mRNA expression of IL-2 and IFN- in liver tissue was then estimated via quantitative real-time PCR (qRT-PCR) that was performed in the Biotechnology Unit, Animal Health Research Institute, Zagazig branch (Egypt)
