GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells 55721318 How It Works: Nickel-NTA beads

How It Works: Nickel-NTA beads retain affinity for His-tagged proteins in the presence of denaturants such as urea or guanidine HCl

Applications: Generation of transgenic crops and model plants such as Arabidopsis

and embryonic stem cells for gene editing applications

4 mg/ml of each protein in the buffer (20mM Tris-phosphate

Trypsin has an optimal operating pH of about 7

GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells 55721318 How It Works: Nickel-NTA beadsGoldBios GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline type Ti plasmid pMP90 (pTiC58DT DNA) that contains the vir genes required for the transfer and integration of T DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin,