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The enzyme was incubated at 37 C for 10 min in a final volume of 0.1 ml in the absence or presence of the extracts diluted as indicated in the figures

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Three key components of ABA signal transduction have been identified in plants: ABA receptors (PYR/PYL/RCAR proteins), negative regulators (PP2C), and positive regulators (SnRK2), together forming a dual negative feedback system (PYR/PYL/RCAR-PP2C-SnRK2) to regulate ABA signal transduction and downstream responses ( NtP2C63 expression in NtPDX2 overexpressing lines, suggesting that the increase in vitamin B 6 levels may enhance H+-ATPase activity, thus promoting stem elongation