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It is made from thin slices of seasoned and marinated meat, stacked into a cone-shaped column, and then roasted on a vertical spit

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The resulting methanol extract liquid was centrifuged and the supernatant containing carotenoid pigment was then quantified by estimating the OD at 462 nm using a microplate reader (BioRAD, USA)

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How gonorrhea spreads between people Gonorrhea is caused by Neisseria gonorrhoeae , a bacterium that infects the wet linings of the body: the urethra, cervix, rectum, throat, and (rarely) the conjunctiva of the eye