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these may be related to specific sequence variants interfering with the action of the DNA methylation machinery ( in vitro studies suggest that the de novo methyltransferases DNMT3A and DNMT3B may have intrinsic preferences for certain flanking sequences (Jurkowska et al., 2011) and that the DNMT3A/DNMT3L complex favors methylation of CpGs distributed periodically and separated by distances of 810 bp ( DIP2B

The immunoblots were developed with enhanced chemiluminescence (ECL) Plus reagent (Amersham, Arlington Heights, IL) and signals were captured by a Fluor-S MultiImager and quantified using Quantity One software (Bio-Rad, Hercules, CA)