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At 80% confluency, cells were harvested using Dulbeccos phosphate buffered saline (DPBS) for washing and incubated with 0.05% trypsin EDTA/DPBS (GIBCO, Invitrogen, Carlsbad, CA) for 2 mins at 37 C to allow detachment from the culture flask

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Zhang, C., Liu, F., Zhai, Y., Ariga, H.O, Yi, N., Liu, Y., Asakura, K., Flytzani-Stephanopoulos, M., and He, H., Angew

Compared with full-time smokers, e-cigarette-only users had 97% lower levels of one toxic chemical, NNAL, that is strongly associated with lung cancer

This mist is often misunderstood as clean water vapor, but it's actually filled with varying amounts of toxic chemicals and carries the same danger as secondhand smoke