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Competent Y2HGold cells were prepared with lithium acetate/PEG and transformed with 200 ng of each BD construct (pGBKT7- NtMYB28 , pGBKT7- NtERF167 , pGBKT7- NtCYC ) or control vectors (pGBKT7-empty and pGBKT7-53)

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[69]According to FDA guidance, factors that may be considered when making the risk-based determination of whether to use a remote assessment tool include establishment location, inspection history, complexity of product and process, and travel restrictions