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A threshold cycle number (CT) for each sample was obtained from a Prime Pro 48 Real-Time PCR System (Techne, United Kingdom) and used to evaluate the relative amount of target mRNA in experimental groups with those of control subjects using the 2 CT method (Livak and Schmittgen, 2001

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Also, with advancing age, the pylorus (the valve between the stomach and duodenum) relaxes and allows extra bile (a compound produced in the liver to aid in digestion) to seep up into the stomach and erode the stomach lining

Theyre not cotton or paper, but cellulose acetate , a type of synthetic plastic derived from wood through a chemical process