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The microbial interactions can be mainly categorized into two types: direct interactions and indirect interactions ( 5.1 Direct microbial interactions Direct interaction refers to the situation where microorganisms directly inhibit or promote the growth and metabolism of neighboring microorganisms through metabolites or other substances they produce (Pierce and Dutton, 2022)
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As the stage performance gained further popularity, Reubens took the character to motion picture with Pee-wee's Big Adventure in 1985, toning down the adult innuendo for the appeal of children
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