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The reaction mixture contained 0.25 l (5 U/l) TaKaRa Ex Taq, 5 l 10 Ex Taq Buffer (20 mM Mg2+ plus), 4 ldNTP mixture (2.5 mM, TaKaRa Bio Inc.), 0.5 M primer, and 50 ng DNA in a total volume of 50 l

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The doctor said his asthma attack symptoms were triggered by second-hand smoke i.e

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Follow the manufacturers operating instructions