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Evidence provided by this study indicated that the application of BS3 during the curing process resulted in substantial changes in the abundance of OTUs on the surface of tobacco leaves, significantly impacting the composition of the microbial community

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Following fermentation, the tobacco leaves are packed into large, tightly wrapped bales known as tercios (made from royal palm bark) or wooden crates

Ex-smokers, those with any self-reported disease, or individuals who were taking medicines were excluded from the study