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Following principal component (PC) analysis, cell clusters were determined via Seurats default graph-based clustering strategy using the FindNeighbors and FindClusters functions

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Table 3 Description of the studies selected about the association of EC use with the consolidation of tobacco and other substance use, and the evidence on EC use as a smoking cessation strategy In Figure 6, the number of studies confirming the limited evidence regarding the use of ECs as a smoking cessation strategy in adolescents (with three cross-sectional studies 56,79,82 ) or as a harm reduction strategy in smoking (with one cross-sectional study 94 ) is presented