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RNAScope FISH (ALI wholemount labeling) ALI cultures on transwell inserts were rinsed with 1 PBS, fixed for 30 min in 3.2% PFA (no sucrose) at room temperature, rinsed twice more with 1 PBS and stored at 4 C

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While the FDA did initially issue a regulation requiring graphic warning labels, those specific warning labels were struck down by the courts

An AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, United States) was used to purify the recovered products according to the manufacturers instructions, followed by 2% agarose gel electrophoresis to check the purity

BMJ Open 11 , e043133 (2021)