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The R package ropls (Version 1.6.2) 45 was used to perform principal component analysis (PCA) and orthogonal least partial squares discriminant analysis (OPLS-DA, confidence level = 0.95, permutations = 200), and a 7-cycle interactive validation was performed to evaluate the stability of the model

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doi: 10.1016/j.ophtha.2011.08.027